A double-strand break within a yeast artificial chromosome (YAC) containing human DNA can result in YAC loss, deletion or cell lethality
نویسندگان
چکیده
منابع مشابه
A double-strand break within a yeast artificial chromosome (YAC) containing human DNA can result in YAC loss, deletion or cell lethality.
Human chromosomal DNA contains many repeats which might provide opportunities for DNA repair. We have examined the consequences of a single double-strand break (DSB) within a 360-kb dispensable yeast artificial chromosome (YAC) containing human DNA (YAC12). An Alu-URA3-YZ sequence was targeted to several Alu sites within the YAC in strains of the yeast Saccharomyces cerevisiae; the strains cont...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1996
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.16.8.4414